InCeption, Innovation, Excellence
InCepix is focused on aiding and furthering our understanding of biology to lead to a betterment of our shared condition.
Tools let us see more and accomplish more. Tools help us to understand more of the world around us. Tools are what we bring with us to tackle the challenges in front of us.
InCepix is committed to bringing new tools for use in the work of improving mankinds understanding of our own intimate biology. With tools in our hands we set out to create a better life for all.
Our EVix tools provide researchers with a means to explore and decipher the complexity of extracellular vesicle biology
Extracellular Vesicles a Tiny Bit of Biology with a Big Impact
Extracellular Vesicle (EV) Research a Quest for a Promise of Hope
Outside of the cell is a busy place. Recent findings are adding to the known complexities that are just outside of the cell membrane.
Cells both healthy and sick, under stress or during development, transformed or neoplastic, release particles into their environment. These particles are varied and complex.
The particles released from cells are varied in their pathway of origination from within the cell. Vesicles are thought to be shed from cells, disgorged from endocytic vesicles, and possibly pinched off in virus like genesis. As the interior of cells is compartmentalized by various vesicle structures, the death of cells can lead also to the release of vesicles into the extracellular space.
While our knowledge of the extracellular space and its contents grows daily, the promise that understanding the role of the multitude of vesicles in this space will provide new pathways for therapy and disease treatment expands as well.
Why the hope? It is because research is making clear that these vesicles can deliver powerful messages to other cells. These vesicles may comprise a communication system between cells both near and far from each other. A communication system that is involved in nearly all aspects of a cells life.
EVs, It gets Complicated Fast
By Definition, an extracellular vesicle has a lipid membrane believed to be similar to the cell membrane. As they can be derived by shedding or involution into a multivesicular body, the lipid membrane is an essential component.
This lipid membrane and their small size imbues them with unique properties. Size of the vesicle determines the amount of membrane bilayer. The smallest vesicles exosomes can be within a range of 40-50 nm in diameter.
In Contrast larger vesicles derived by shedding or remnants of apoptotic cells can range up to 500 to 1000 nm in diameter
Diverse and Complex
Most EV preparations are quite diverse. While it may be possible to isolate a certain sized population, remember it is still a distribution around an average value
It is most likely that any purified preparation will still contain vesicles which may be unwanted.
Remember this is just with size. The density, payload, surface properties and other features of vesicles quickly raise the amount of diversity in a preparation.
EVix Tools: Antigen Specific Capture and Detect
EVix tools provide monoclonal antibody capture of EVs. EVs can be captured onto beads or in microwells. The captured EVs can then be detected by selecting an appropriate detection antibody.
EVs can be captured by tetraspanin specific antibodies. Anti CD9, CD63, and CD81 appear to be prevalent on EVs, and can be detected on EVs which are larger than traditional exosomes but also on exosomes both large and small.
Anitgens which are not tetraspanins can also be used to capture EVs. Detecting bound EVs can be done with anti tetraspanin antibodies as well as other non tetraspanin antibodies.
EVix Tools for Characterizing and Tracking EVs
Use EVix Tools to capture and detect EVs. Track antigen specific EVs in your preparations and purification work flow.
Detect Tetraspanin Positive EVs in a Variety of Samples
Track tetraspanin positive vesicles, during purification or isolation
Compare EV Populations from Different Sample Sources
Monitor EV populations derived from different conditions or samples
Search for Specific Antigen Positive Populations
Identify antigen specific members in an EV population
EVix ELISA Kits
Our EVix ELISA kits are ready to go. Complete and accurate, ready to assay your EV preparation. High sensitivity, flexibility and highly reproducible.
Select the capture antigen for your application. Urine EVs express high levels of CD10 (Neprilysin), low levels of CD326 (EpCAM) as well as other antigens. Capture plates directed against these antigens will capture antigen positive EVs which can be detected by either anti tetraspan antibodies, or other antigen specific antibodies.
Low level antigen expressing populations can be captured by direct antigen capture, whereas they may go undetected if captured by a high level antigen.
EVix Capture Plates
EVix capture plates are available ready for use. Select from your antigen of choice. We have an extensive list of antigen specific capture plates.
The capture plate is the heart of the EVix ELISA kit, and a combination of capture plates and detection reagents are available in complete kit form, however if you have your own detection reagents you can select a capture plate directed against your selected capture antigen.
Capture plates come optimally coated with monoclonal capture antibody and can used upon receipt following a single wash prior to capture. Each capture plate is provided in a format of 12 strips of 8 wells each. Depending upon assay size, strips can be utilized independently or the entire plate can be used in total.
EVix Capture Beads
EVix capture beads are available for flow cytometry applications. Matched to capture plate antibody coatings, EVix beads allow for depletion or capturing of EVs. Capturing of antigen specific EVs can be accomplished by incubation of EVix beads with an EV sample. Adjusting bead concentrations to EV content can be used to remove antigen specific EVs from a population, or to capture EVs for further antigen characterization.
Payload analysis of EVs can be done by capturing antigen specific EVs and employing downstream using PCR or western blot analysis on the antigen specific EVs.
EVix capture beads are available with anti tetrspanin antibody coatings as well as other relevant antigen directed antibodies.
Epitope specificity is matched for both beads and capture plates allowing for use of both tools in concert to examine EV populations.
EVix Detection Reagents
EVix detection reagents are available to detect EVs retained on capture plates or EVix beads. The EVix detection reagents are all certified and highly purified reagents that are available in a number of fluorescent and biotinylated tagged formats.
Biotinylated detection antibodies can be used in conjunction with EVix capture plates for setting up your own ELISA assays.
EVix detection antibodies provide researchers a wide pallet of tools to use in phenotyping EV populations. The detection reagents can be selected for your own specific EV preparations and needs.
EVix ELISA tools reveal phenotype differences in EV populations obtained from cell culture
EV samples concentrated by ultra filtration reveal fundamental differences using EVix ELISA kits. Colon cancer cell lines SW480 and SW620 were grown in culture and supernatants were concentrated and analyzed for detection of antigen specific EV populations.
EVix ELISA tools reveal Neprilysin and EpCAM positive EVs in normal human urine.
Urine derived EV samples concentrated by ultra filtration reveal fundamental differences using EVix ELISA kits. Screening for non tetraspanin antigens revealed populations which were Neprilysin (CD10) and EpCAM (CD326) positive.