SurVeyix Pre-dispensed Biotinylated Antibody Plates
Research Use Only Not for Diagnostic Use
Examine antigen phenotypes on cells or other biological samples simply with plates of predispensed and preserved monoclonal antibodies. Each well contains preserved antibody that when diluted in 100 ul of sample or buffer will yield working concentration of antibody to bind to antigen on cell surface or in other preparations.
The predispensed antibody plates allow for surveying or screening hundereds of antigens without the labor involved in ordering and diluting hundreds of antibodies for each assay. SurVeyix microwell plates come ready for use. The plate map identifies each well and corresponding antibody. Add 100 ul of cell suspension or other sample type directly to the well and the antibody is reconstituted to a working concentration of 2 ug/ml. The biotinylated antibody panels contain biotinylated monoclonal antibodies which can be captured or detected by streptavidin reagents.
InCepix pre-dispensed biotinylated antibody plates allow for deep phenotyping of cells via flow cytometry. Samples can be directly added to the SurVeyix plate and incubated prior to transfer to individual flow tubes for washing and secondary staining. Wide coverage of surface expressed molecules with a focus on immunological species is provided in our first SurVeyix plates. Future plates are anticipated focusing on additional surface molecules.
Shown above are examples of cells stained by reconstituted antibody from SurVeyix plates. A mixed population of cells (equal number 4 x 10^6/mlof HPB-Null, Raji, and Nalm-6) were suspended in FACs buffer and dispensed in 50 ul volumes into individual wells of SurVeyix plate. After 30 min incubation, contents were removed and processed by washing and incubation with SA-PE followed by washing and analysis.
Additional histograms of the SurVeyix wells are shown in the following PDF files. SurVeyix Note that the mixed cell population results in Triple positive, triple negative, and single and double positive signals, depending upon antibody specificity.
Plate Map for SurVeyix Pre-Dispensed Biotinylated Antibody Plate SVX-001 (115 antibodies) is shown below. Each well is identified by location and antibody content. For detailed performance specifications of each antibody please consult the listing below which links catalogue number to each antibody. Going to the Ancell catalogue and entering the catalogue number in the search field will yield detailed information for each antibody.
Antibodies are predispensed into 96 well tissue culture plates in a proprietary preservative which preserves antibody activity during storage at 4 C. Reconstitution of active antibody occurs with addition of sample. For example for flow cytometry, cells suspended in FACs binding buffer can be added directly into the plate wells. Each well receives a total of 100 ul of sample which reconstitutes antibody to a working concentration in each well.
Screen 115 antigens in a single assay.
Survey binding of 115 different biotinylated antibodies to target cells.
Add cells directly to wells and incubate. Antibody present in wells binds to relevant antigens on cells if antigen is present. Remove from well and further assay with washing and SA secondary reagents in FACs tubes, or if a plate reader is available, finish assay in survey plate and read directly.
High Resolution Finger Printing
Track cell expression of 115 antigens in your cell populations. Two color analysis is possible with cells pretreated with different fluorochromes.
Run assay directly in antibody containing wells. No special instrument or array reader required. Go directly from plate to FACs with a plate sampler.
Compare differences between cell populations. Examine impact of experimental treatments on antigen expression.
Quantify antigen profiles between different samples and clinical conditions. The absence or presence of antigens can be distinctive and related to cell source or physiological conditions..
Cell surface antigen targets
Examine cell surface expression patterns of 115 different unique molecules. Main focus on immune related surface molecules.
Build expression data bases on cell population antigen expression patterns
SurVeyix plates allow creation of powerful and large data bases on the surface proteome. Peripheral blood mononuclear cells can be extensively phenotyped in a simple assay.
SurVeyix plates contain biotinylated antibody conjugates predispensed in the individual wells of a 96 well microplate.
Each well contains a unique antibody. The antibody is preserved in a concentrated state and is resuspended upon addition of buffer. The maps of the plates indicate which well contains which antibody.
Reconstitution of well with 50 ul creates a 4 ug/ml antibody concentration in the 50 ul volume. Adding 100 ul of cells in buffer to well results in a 2 ug/ml antibody concentration and is ideal for most cell surface staining applications.
Surveyix plates are stable for at least 6 months when kept at 4C sealed in foil pouch.
Order SU-001. 115 different biotinylated antibodies preserved and ready for reconstitution in 2 96 well plates.